Composite
Part:BBa_K2481106:Design
Designed by: Erin Kelly Group: iGEM17_Lethbridge_HS (2017-10-27)
indB expression construct
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 701
Illegal BamHI site found at 852 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1135
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Coding sequences and regulatory elements were optimized for expression in E. coli
Source
DNA was synthesized by IDT and the coding sequence of indB was derived from Uniprot