Composite

Part:BBa_K2481106:Design

Designed by: Erin Kelly   Group: iGEM17_Lethbridge_HS   (2017-10-27)


indB expression construct


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 701
    Illegal BamHI site found at 852
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1135
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Coding sequences and regulatory elements were optimized for expression in E. coli


Source

DNA was synthesized by IDT and the coding sequence of indB was derived from Uniprot

References